BS ISO 22104-2021 水质.微囊藻毒素的测定.液相色谱法和串联质谱法(LC-MSMS)
基本信息
标准号:ISO 22104:2021
其他编号:ISO 22104-2021
发布单位或类别:国际组织-国际标准化组织(IX-ISO)
发布日期:2021-07-01
实施日期:
废止日期:
CCS分类:
ICS分类:
13.060.50 水质 – 水的化学物质检验
适用范围/摘要
本文件规定了饮用水和淡水样品中12种微囊藻毒素变体(微囊藻毒素LR、-LA、-YR、-RR、-LY、-WR、-HtyR、-HilR、-LW、-LF、[Dha7]-微囊藻毒素LR和[Dha7]-微囊藻毒素RR)在0.05μg/l至1.6μg/l之间的定量方法。该方法可用于进一步测定微囊藻毒素,前提是对每种微囊藻毒素的色谱和质谱检测分析条件进行了测试和验证。使用内部标准校准,通过LC-MS/MS对样品进行分析。
这种方法是基于性能的。允许实验室修改该方法,即。
g、 增加低干扰样品的直接流动注射量,或稀释样品以增加工作范围上限,前提是满足本方法中的所有性能标准。
附录A中描述了用高分辨率质谱法(HRMS)检测微囊藻毒素,作为串联质谱法(MS/MS)的替代方法。
附录B中描述了一种基于在线固相萃取与液相色谱相结合的自动样品制备方法。
当仪器灵敏度不足以通过直接流动注射达到方法检测限时,附录C中描述了固相萃取净化和浓缩步骤。
This document specifies a method for the quantification of twelve microcystin variants (microcystin-LR, -LA, -YR, -RR, -LY, -WR, -HtyR, -HilR, -LW, -LF, [Dha7]-microcystin-LR, and [Dha7]-microcystin-RR) in drinking water and freshwater samples between 0,05?μg/l to 1,6?μg/l. The method can be used to determine further microcystins, provided that analytical conditions for chromatography and mass spectrometric detection has been tested and validated for each microcystin. Samples are analysed by LC-MS/MS using internal standard calibration.
This method is performance based. The laboratory is permitted to modify the method, e.g. increasing direct flow injection volume for low interference samples or diluting the samples to increase the upper working range limit, provided that all performance criteria in this method are met.
Detection of microcystins by high resolution mass spectrometry?(HRMS) as an alternative for tandem mass spectrometry?(MS/MS) is described in Annex?A.
An alternative automated sample preparation method based on on-line solid phase extraction coupled to liquid chromatography is described in Annex?B.
When instrumental sensitivity is not sufficient to reach the method detection limits by direct flow injection, a solid phase extraction clean-up and concentration step is described in Annex?C.
研制信息
起草单位:
起 草 人:
归口单位:
ISO/TC 147/SC 2